International Journal of Chemical and Biochemical Sciences (ISSN 2226-9614)[/vc_column_text][/vc_column][/vc_row]
VOLUME 25(19) (2024)
The Effect of Adding Chitosan Nanoparticles Loaded with Sidr Leaf Extract (Ziziphus spina-christi) (CPNs SLE) in Diet on Pangasius sp. Infected Aeromonas hydrophila
Slamet Budi Prayitno1, Annisa Syahida Aulia1, Siti Dinda Chrisnawat2, Desrina1, Sarjito Sarjito1*
1Department of Aquaculture, Faculty of Fisheries and Marine Science, Universitas Diponegoro, Semarang, 50275, Indonesia.
2Department of Aquatic Resources, Faculty of Fisheries and Marine Science, Universitas Diponegoro, Semarang, 50275, Indonesia.
Abstract
One of the bacterial types that affects freshwater fish is Aeromonas hydrophila. These bacteria cause the occurrence of Motile Aeromonas Septicemia (MAS) disease. This study investigates the impact and optimal dosage of chitosan nanoparticles (CNPs) loaded with sidr leaves extract (SLE) on clinical manifestations, growth, hematological parameters, and survival rates of Pangasius sp. infected A. hydrophila. The experimental design employed a Completely Randomized Design with five treatments and three replications, utilizing 150 fish with an average length of 7-9 cm. Treatments included the addition of 10 ppm tetracycline (K+) in the diet and CNPs SLE with concentrations A (0 ppm), B (450 ppm), C (500 ppm), and D (550 ppm). The results demonstrate that incorporating CNPs SLE into the diet yields beneficial effects on infected Pangasius sp., leading to faster recovery from clinical symptoms, enhanced growth rates, and increased survival rates of up to 85%. The optimal dosage of CNPs SLE for Pangasius sp. infected A. hydrophila in the feed is determined to be 500 ppm.
Keywords: Sidr leaf, A. hydrophila, Chitosan Nanoparticles. Pangasius sp., blood profile
Full length article – PDF *Corresponding Author, e-mail: sarjito@live.undip.ac.id Doi # https://doi.org/10.62877/134-IJCBS-24-25-19-134