International Journal of Chemical and Biochemical Sciences (ISSN 2226-9614)[/vc_column_text][/vc_column][/vc_row]
VOLUME 26(20) (2024)
Electrospun Poly-Lactic Acid Nanofibers as a scaffold for Regenerative Endodontics purposes: Fabrication and Characterization
Mai S Ghoneim*a, Radwa M. A. Abd-Elal b, Ingy M. Kataia c d, Ashraf M Abu-Seida e, Abeer H Mahran f
a PhD candidate, Department of Endodontics, Faculty of Dentistry, Ain Shams University, Cairo, Egypt
b Pharmaceutics and Drug Manufacturing Department, Modern University for Technology and Information (MIT), Cairo 11571, Egypt
c Department of Endodontics, Faculty of Dentistry, The British University in Egypt (BUE), El Sherouk City, Egypt
d Restorative and Dental Materials Department, National Research Centre, Giza, Egypt
e Professor, Veterinary Surgery Department, Faculty of Veterinary Medicine, Cairo university, Giza, Egypt
f Professor, Department of Endodontics, Faculty of Dentistry, Ain Shams University, Cairo, Egypt
Abstract
Poly (lactic acid) (PLA), which is a polyester, undergoes hydrolytic degradation into lactic acid, a natural metabolic byproduct, making it well-suited for medical applications. PLA nanofibers have expanded the potential applications of PLA scaffolds, particularly in regenerative medicine and drug delivery. These nanofibrous scaffolds offer several advantageous features, including a high surface area, the ability to mimic the architecture of the native extracellular matrix, and tunable mechanical properties (factors that are crucial when designing scaffolds for specific organ systems). This study deals with the synthesis, characterization, and evaluation of PLA nanofibrous scaffold. For the fabrication. Polylactic acid (PLA) polymer solution at a concentration of 25 wt% was processed into Fibres. Characterization used in the current study included scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), degradation tests. The antimicrobial properties were tested against Porphyromonas gingivalis (Pg) and Enterococcus faecalis (Ef). SEM imaging showed Fibres with submicron diameters, and FTIR spectra of nanofibers showed that PLA was evident by the presence of the characteristic peaks. Antimicrobial activity was confirmed via agar diffusion assays, showing evidence of antimicrobial activity against the tested bacteria. These findings suggest that PLA scaffolds could serve as a biologically safe system for further use in regenerative endodontics.
Keywords: Electro spinning, poly lactic acid, nanofiber, pulp regeneration, scaffold
Full length article *Corresponding Author, e-mail: mai_ghoneim@yahoo.com https://doi.org/10.62877/22-IJCBS-24-26-20-22